Central role of mass spectrometry: ionization sources, mass analyzers, different types of mass spectrometers; Quantitative proteomics - stable isotope labelling by amino acids in cell culture (SILAC), isotope-coded affinity tag (ICAT), isobaric tagging for relative and absolute quantitation (iTRAQ)
Functional proteomics: Recombinational cloning, Interactomics techniques to study protein-protein interactions, yeast two-hybrid, immunoprecipitation, protein microarrays, Nucleic Acid Programmable Protein Array (NAPPA)
Label-free nanotechnologies in proteomics, Surface Plasmon Resonance (SPR); Modificomics: understanding post-translational modifications; Structural proteomics; Bioinformatics in proteomics; Challenges and future prospects of proteomics research
Proteomics course will cover several basic concepts of various technologies but emphasis will be more on software's and hands-on sessions/ data analysis.
Introduction to Proteomics: Tools for the New Biology, D.C. Liebler, Humana Press, 2002.
Principles of Proteomics, R.M. Twyman, Bios Scientific Pub., 2004.
Proteomics for Biological Discovery, T.D. Veenstra, J.R. Yates III, John-Wiley & Sons, Hoboken, New Jersey, USA; 2006.
Protein Biochemistry and Proteomics (The Experimenter Series), R. Hubert, Academic Press, 2006.
Proteomics in Practice: A Guide to Successful Experimental Design, R. Westermeier, T. Naven, H-R. Hopker, Wiley-VCH, 2008.
Proteomics: A Cold Spring Harbor Laboratory Course Manual, A.J. Link and J. LaBaer, Cold Spring Harbor Laboratory Press, 2009.